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Arriba v2.2.0

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@suhrig suhrig released this 16 Jan 13:03
· 50 commits to master since this release
  • improved detection of internal tandem duplications
  • better sensitivity for the detection of viral integration sites
  • inclusion of additional ~4500 viruses into screening, including rare strains of cancer-associated viruses (requires rebuild of STAR index)
  • viral contigs were renamed to be compliant with the SAM format specification (requires rebuild of STAR index)
  • support for mm39/GRCm39
  • utility scripts (see also manual):
    • quantify virus expression
    • convert Arriba's custom output format to VCF
    • extract fusion-supporting alignments into separate mini-BAM
    • running Arriba on a prealigned BAM file and realigning only the fusion candidate reads saves ~80% of the CPU time compared to a complete realignment (useful when the alignments were generated by an old STAR version or by a different aligner such as HISAT2)
  • polishing of fusion visualizations created by draw_fusions.R and new features:
    • all transcripts can be drawn at the same scale if desired (--fixedScale)
    • circos plots have same size across all pages
    • set PDF title and print as header on every page (--sampleName)
    • fine-grained control over region to draw for intergenic breakpoints (--showIntergenicVicinity)
    • choose a different font (--fontFamily)
    • better scaling for coverage track
  • more fixes for prediction of reading frame
  • better warnings and error messages
  • updated STAR to version 2.7.10a, which fixes malformed chimeric alignments for paired-end reads with small insert size
  • updated dependencies (HTSlib, libdeflate)