Human Friendly Read ID

Each MinION sequenced read is assigned a unique 128-bits number (aka read_uuid) by the MinKNOW software. The read_uuid is reported as 32 hexidecimal digits (e.g. 27bc311c-87ef-496b-9153-7f198ed69e63). To make tracking reads alignment easier in IGV browser, we assigned a human-friendly read id composed of 7 elements as follow:

Element	Description	Examples
1	experiment id	WTD09
2	unique running serial number from 1 assigned to each read	1
3	1D or 2D status of read	2D
4	Pass or Fail status of read	P
5	Read code	1 = Read template sequence is present 2 = Read complement sequence is present 3 = Both read template and complement sequence are present
6	Read sequence code	T=Template C=Complement M=Merged consensus 2D sequence
7	Read sequence length prefixed with 'L'	L23284

To facilitate tracking these IDs back to the read_uuid, each run/experiment has an associated hash table (<experiment>-hash.xls). The first line of the hash table contains the columns header prefixed by '#'. Each line contains 7 columns as follow:

Column	Description	Examples
1	read_uuid	UUID assigned by MinKNOW software
2	fast5	.fast5 file where read sequences is extracted from
3	PassFail	P=Pass, F=Fail
4	1D_2D	1D read(s) or 2D-reads
5	template_hash_id	human-friendly read id for read's template sequence
6	complement_hash_id	human-friendly read id for read's template sequence
7	2d_hash_id	human-friendly read id for read's template sequence