In this study we recreated an experiment published by Hablitz et al., 2020, which analysed the circadian control over the perivascular polarization of the water channel protein AQP-4. In our experiment, we were able to replicate the results but also approached the data analysis in a different manner which lead us to question the method used in Hablitz et al., publication. We also found, using mice with a abnormal circadian clock (total Per2 knockouts), that these mutants may have a more active glymphatic system than wild type mice. This finding may be a good starting point for further studies involving the glymphatic system and its relationship with the circadian rhythm.
This repository contains data from fluorescent microscopy and the relevant scripts for its processing and analysis.
The notebook has a few examples for using the functions.