"RuntimeError: ConnectionPool is closed" when registration

[azzhu]

Bug report

Description of the problem

Data information：
total 16 tiles
the dimensions of each tile is [1920,1920,1099]
datatype:uint16
the pixel resolution is [0.23,0.23,0.42]
sum size of two rounds is about 1T
each round has four channels "c0,c1,c2,c3"
the dapi channel is "c0"

When I skipped other modules and just run registration, an error had occurred:

Log file(s)

Environment

• EASI-FISH Pipeline version: [dev]
• Nextflow version: [23.04.1]
• Container runtime: [Singularity]
• Platform: [Slurm]
• Operating system: [CentOS 7.6]

Additional context

(Add any other context about the problem here)